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analytical lab news1 News #1: Chondroitin
analytical lab news2 News #2: Glucosamine
analytical Lab news3 News #3: L-Carnitine
analytical Lab news4 News #4: Chondroitin & Glucosamine in Pet Food
analytical lab new5 News #5: Single Lab Validation
analytical lab news6 News #6: Identification and Quantification of Oversulfated Chondroitins
   
   
   
Activities
Member of AOAC method Committee for Dietary Supplement
Active participant in Dietary Supplement Task Force
Official contractor for NIH through BPA program (Blanket Purchase Agreement)
 
Completed Single Lab Validation of Enzymatic-HPLC Method for Chondroitin Sulfate Assay funded by NIH, and the paper is going to be published on Journal of AOAC
 
Developed Pre-Column Derivatization Method for Glucosamine Assay funded by NIH, and the paper have been published on Journal of AOAC
 
 
 
 
 
 
 
 
 
Derivative - HPLC Method for Glucosamine Testing

Glucosamine: Testing of glucosamine raw material and simple formulas can be done by HPLC with EC (Electrochemical) or UV detection at 195-200nm wavelength:
  • UV detection--As any experienced chromatographer knows, at this low wavelength the method could face challenging interference and high background noise. If your contract laboratory uses UV detection for glucosamine testing, you need to double-check the reliability of reported results.

  • EC detection-The required experience in proper care/operation of an EC detector and narrow application of this technique could make this assay a relatively unaffordable one in many laboratories. In addition, the Redox reaction on an electrode surface can be very complicated and irreproducible. It is much less rugged than a UV detector in general.

Testing of a wide variety of glucosamine products, especially complex formulas or low levels of glucosamine (as low as <0.1%, w/w), a pre-column derivatization HPLC method used at Analytical Laboratories In Anaheim, Inc. has been extremely efficient, sensitive and reliable:

  • Pre-column derivatization HPLC-Method has been used in testing raw materials, dosage forms and animal feed containing trace levels glucosamine. The principle of this validated method: the lack of chromophores in glucosamine is overcome by forming an adduct that gives UV absorption at a higher wavelength range 240-260 nm. This reduces background noise and interference, and increases the signal to noise level tremendously. In addition, the derivative increases the retention of glucosamine with a reversed-phase HPLC system. (See chromatograms below.)

testing data diagram

  testing data diagram

Fig. 1. (A) standard at 15.0 ppm concentration, (B) a feed sample at 0.11% trace level
 
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